Journal: PLoS ONE

Article Title: Extracts of Strawberry Fruits Induce Intrinsic Pathway of Apoptosis in Breast Cancer Cells and Inhibits Tumor Progression in Mice

doi: 10.1371/journal.pone.0047021

Figure Lengend Snippet: CEM and T47D cells were treated with increasing concentrations of MESB (0.1, 0.2, 0.5 and 1 mg/ml) and cells were harvested after 48 or 72 h of treatment and subjected to trypan blue, MTT and LDH assays. A. Determination of cell viability by trypan blue assay in CEM cells. B. Evaluation of cell proliferation by MTT assay in CEM cells. C. Bar diagram showing release of lactate dehydrogenase following MESB treatment in CEM cells. D. Assessment of cell viability using trypan blue assay in T47D cells. E. Determination of cell proliferation by MTT assay in T47D cells after MESB treatment. F. LDH assay showing release of lactate dehydrogenase in T47D cells following addition of MESB. In each panel, error bars were calculated based on results obtained from minimum of three independent experiments. In all panels, *p<0.05, **p<0.005, and ***p<0.0005.

Article Snippet: Current study investigates the anticancer activity of the methanolic extract of strawberry (MESB) fruits in leukaemia (CEM) and breast cancer (T47D) cell lines ex vivo, and its cancer therapeutic and chemopreventive potential in mice models.

Techniques: MTT Assay, Lactate Dehydrogenase Assay

Journal: PLoS ONE

Article Title: Extracts of Strawberry Fruits Induce Intrinsic Pathway of Apoptosis in Breast Cancer Cells and Inhibits Tumor Progression in Mice

doi: 10.1371/journal.pone.0047021

Figure Lengend Snippet: Whole cell extracts ( A-C ) and cyotosolic extracts ( D ) were prepared from T47D cells following treatment with MESB (0, 0.1, 0.4, 0.7 mg/ml for 48 h). Western blotting studies were performed using primary antibodies against (A) MCL-1, BCL-xL, BAX and BID, (B) p53, MDM2, p73 and PARP1, (C) SMAC/DIABLO, CYTOCHROME C, APAF1, CASPASE 3 and CASPASE 9, (D) SMAC/DIABLO and CYTOCHROME C. In panels A-C, TUBULIN was used as an internal loading control, while in D, ACTIN was used.

Article Snippet: Current study investigates the anticancer activity of the methanolic extract of strawberry (MESB) fruits in leukaemia (CEM) and breast cancer (T47D) cell lines ex vivo, and its cancer therapeutic and chemopreventive potential in mice models.

Techniques: Western Blot, Control

Journal: BMC Complementary Medicine and Therapies

Article Title: Cytotoxicity, acute and sub-chronic toxicities of the fruit extract of Tetrapleura tetraptera (Schumm. & Thonn.) Taub. (Fabaceae)

doi: 10.1186/s12906-022-03659-1

Figure Lengend Snippet: Chemical structures of compounds 1 to 9 isolated from TTF extract. 1: (3 R , 4S)-3,4-dimethyloxetan-2-one; 2: luteolin, 3: stigmasterol; 4; 3- O -[6’- O -undecanoyl- β - D -glucopyranosyl]stigmasterol; 5: oleanan-12-en-3- β - O - D -glucopyranoside; 6: 3- O - β - D -glucopyranosyl-(1 → 6)- β - D -glucopyranosylurs-12-en-28-oic acid; 7: 3- O - β - D -glucopyranosyl-(1 → 3)- β - D -glucopyranosyl-27-hydroxyolean-12-ene-28-oic acid; 8: methyl- O - β - D -glucopyranoside; 9: β - D -fructofuranosyl-(2 → 1)- β - D -glucopyranoside

Article Snippet: Furthermore, the methanol crude extract of TTF fruits and four of its isolated constituents had antiproliferative activity towards drug-susceptible and multidrug-resistant cancer lines, with IC 50 values for the crude extract and the most active compound (olean-12-en-3- β-O - D -glucopyranoside) ranging from 10.27 μg/mL (in CCRF-CEM leukemia cells) and 23.61 μg/mL (against HCT116 p53 −/− colon adenocarcinoma cells) and from 4.76 μM (against CCRF-CEM cells) to 12.92 μM (against HepG2 hepatocarcinoma cells) [ ].

Techniques: Isolation

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Acute and Subacute Toxicities of Psidium guajava Methanolic Bark Extract: A Botanical with In Vitro Antiproliferative Potential

doi: 10.1155/2019/8306986

Figure Lengend Snippet: Effect of oral administration of the Psidium guajava extract on body weight changes of female rats during 28 days of the subacute toxicity study.

Article Snippet: The methanolic bark extract of P. guajava revealed considerable cytotoxic activity against CEM/ADR5000 cells with a 50% inhibitory concentration (IC50) of 1.29 μ g/mL, against HCT 116 cells (p53+/+) with an IC50 of 18.63 μ g/mL, and against several other sensitive and resistant cells with IC50 values up to 62.64 μ g/mL (towards MDA-MB-231 cells) [ ].

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Acute and Subacute Toxicities of Psidium guajava Methanolic Bark Extract: A Botanical with In Vitro Antiproliferative Potential

doi: 10.1155/2019/8306986

Figure Lengend Snippet: Effect of oral administration of the Psidium guajava extract on body weight changes of male rats during 28 days of the subacute toxicity study.

Article Snippet: The methanolic bark extract of P. guajava revealed considerable cytotoxic activity against CEM/ADR5000 cells with a 50% inhibitory concentration (IC50) of 1.29 μ g/mL, against HCT 116 cells (p53+/+) with an IC50 of 18.63 μ g/mL, and against several other sensitive and resistant cells with IC50 values up to 62.64 μ g/mL (towards MDA-MB-231 cells) [ ].

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Acute and Subacute Toxicities of Psidium guajava Methanolic Bark Extract: A Botanical with In Vitro Antiproliferative Potential

doi: 10.1155/2019/8306986

Figure Lengend Snippet: Effect of oral administration of the Psidium guajava extract on food intake of female rats during 28 days of the subacute toxicity study. ∗ indicates significant values at p < 0.05.

Article Snippet: The methanolic bark extract of P. guajava revealed considerable cytotoxic activity against CEM/ADR5000 cells with a 50% inhibitory concentration (IC50) of 1.29 μ g/mL, against HCT 116 cells (p53+/+) with an IC50 of 18.63 μ g/mL, and against several other sensitive and resistant cells with IC50 values up to 62.64 μ g/mL (towards MDA-MB-231 cells) [ ].

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Acute and Subacute Toxicities of Psidium guajava Methanolic Bark Extract: A Botanical with In Vitro Antiproliferative Potential

doi: 10.1155/2019/8306986

Figure Lengend Snippet: Effect of oral administration of the Psidium guajava extract on food intake of male rats during 28 days of the subacute toxicity study. ∗ indicates significant values at p < 0.05.

Article Snippet: The methanolic bark extract of P. guajava revealed considerable cytotoxic activity against CEM/ADR5000 cells with a 50% inhibitory concentration (IC50) of 1.29 μ g/mL, against HCT 116 cells (p53+/+) with an IC50 of 18.63 μ g/mL, and against several other sensitive and resistant cells with IC50 values up to 62.64 μ g/mL (towards MDA-MB-231 cells) [ ].

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Acute and Subacute Toxicities of Psidium guajava Methanolic Bark Extract: A Botanical with In Vitro Antiproliferative Potential

doi: 10.1155/2019/8306986

Figure Lengend Snippet: Effect of oral administration of the Psidium guajava extract on the relative weight of organs and weight gain.

Article Snippet: The methanolic bark extract of P. guajava revealed considerable cytotoxic activity against CEM/ADR5000 cells with a 50% inhibitory concentration (IC50) of 1.29 μ g/mL, against HCT 116 cells (p53+/+) with an IC50 of 18.63 μ g/mL, and against several other sensitive and resistant cells with IC50 values up to 62.64 μ g/mL (towards MDA-MB-231 cells) [ ].

Techniques: Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Acute and Subacute Toxicities of Psidium guajava Methanolic Bark Extract: A Botanical with In Vitro Antiproliferative Potential

doi: 10.1155/2019/8306986

Figure Lengend Snippet: Effect of oral administration of the Psidium guajava extract on hematological parameters.

Article Snippet: The methanolic bark extract of P. guajava revealed considerable cytotoxic activity against CEM/ADR5000 cells with a 50% inhibitory concentration (IC50) of 1.29 μ g/mL, against HCT 116 cells (p53+/+) with an IC50 of 18.63 μ g/mL, and against several other sensitive and resistant cells with IC50 values up to 62.64 μ g/mL (towards MDA-MB-231 cells) [ ].

Techniques: Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Acute and Subacute Toxicities of Psidium guajava Methanolic Bark Extract: A Botanical with In Vitro Antiproliferative Potential

doi: 10.1155/2019/8306986

Figure Lengend Snippet: Effect of oral administration of the Psidium guajava extract on urinary biochemical parameters.

Article Snippet: The methanolic bark extract of P. guajava revealed considerable cytotoxic activity against CEM/ADR5000 cells with a 50% inhibitory concentration (IC50) of 1.29 μ g/mL, against HCT 116 cells (p53+/+) with an IC50 of 18.63 μ g/mL, and against several other sensitive and resistant cells with IC50 values up to 62.64 μ g/mL (towards MDA-MB-231 cells) [ ].

Techniques: Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Acute and Subacute Toxicities of Psidium guajava Methanolic Bark Extract: A Botanical with In Vitro Antiproliferative Potential

doi: 10.1155/2019/8306986

Figure Lengend Snippet: Effect of oral administration of the Psidium guajava extract on serum biochemical parameters.

Article Snippet: The methanolic bark extract of P. guajava revealed considerable cytotoxic activity against CEM/ADR5000 cells with a 50% inhibitory concentration (IC50) of 1.29 μ g/mL, against HCT 116 cells (p53+/+) with an IC50 of 18.63 μ g/mL, and against several other sensitive and resistant cells with IC50 values up to 62.64 μ g/mL (towards MDA-MB-231 cells) [ ].

Techniques: Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Acute and Subacute Toxicities of Psidium guajava Methanolic Bark Extract: A Botanical with In Vitro Antiproliferative Potential

doi: 10.1155/2019/8306986

Figure Lengend Snippet: Liver (L) and kidney (K) sections of female rats showing effects of oral administration of the Psidium guajava extract over 28 days; L0, K0: control group; L1, K1: 250 mg/kg; L2, K2: 500 mg/kg; L3, K3: 1000 mg/kg. Indicators: A, branch of the hepatic portal vein; B, hepatocytes; c, leukocyte infiltration inflammation; s, sinusoid; v, centrolobular vein; G, glomerulus; EU, urinary space.

Article Snippet: The methanolic bark extract of P. guajava revealed considerable cytotoxic activity against CEM/ADR5000 cells with a 50% inhibitory concentration (IC50) of 1.29 μ g/mL, against HCT 116 cells (p53+/+) with an IC50 of 18.63 μ g/mL, and against several other sensitive and resistant cells with IC50 values up to 62.64 μ g/mL (towards MDA-MB-231 cells) [ ].

Techniques: Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Evaluation of Acute and Subacute Toxicities of Psidium guajava Methanolic Bark Extract: A Botanical with In Vitro Antiproliferative Potential

doi: 10.1155/2019/8306986

Figure Lengend Snippet: Liver (L) and kidney (K) sections of male rats showing effects of oral administration of the Psidium guajava extract over 28 days; L0, K0: control group; L1, K1: 250 mg/kg; L2, K2: 500 mg/kg; L3, K3: 1000 mg/kg. Indicators: A, branch of the hepatic portal vein; B, hepatocytes; s, sinusoid; v, centrolobular vein; G, glomerulus; EU, urinary space; Ep, hepatic portal space; Cb, bile duct; P, proximal convoluted tubule.

Article Snippet: The methanolic bark extract of P. guajava revealed considerable cytotoxic activity against CEM/ADR5000 cells with a 50% inhibitory concentration (IC50) of 1.29 μ g/mL, against HCT 116 cells (p53+/+) with an IC50 of 18.63 μ g/mL, and against several other sensitive and resistant cells with IC50 values up to 62.64 μ g/mL (towards MDA-MB-231 cells) [ ].

Techniques: Control